Positive and Negative Ion Fast Atom Bombardment Mass Spectrometry of Glycosphingolipids.
Masanobu ARITA*,
Masao IWAMORI*,
Tetuo HIGUCHI** and
Yoshitaka NAGAI*
*Department of Biochemistry, Faculty of Medicine, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113
**Scientific Instrument Division, JEOL Ltd. Nakagami-cho, Akishima, Tokyo 196
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undefined undefinedHigh molecular weight gangliosides (GD1a, GD1b, GT1a, and GT1b) and neutral glycosphingolipids (Forssman antigen, globoside, and CTH) containing various fatty acids were analyzed by positive and negative ion fast atom bombardment mass spectrometry (POS-FAB-MS and NEG-FAB-MS) without any derivatization. NEG-FAB-MS of the molecules gave intense peaks of the molecular ion species, (M-H)C, and many fragment ions useful for the elucidation of carbohydrate structure were also detected with significant intensity. The fragment ions were assessed to be cleaved at the glycosidic linkages sequentially from the non-reducing end with or without the ceramide portion and it was possible to distinguish structural isomers having different binding positions of sialic acid. All molecular ion species of neutral glycosphingolipids with various fatty acids were detected at intensities that were in accordance with the relative abundance of the fatty acids.
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undefined undefined1 This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and a grant from the Toray Science Foundation.
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